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Sample GSM322070 Query DataSets for GSM322070
Status Public on Sep 18, 2008
Title Zebrafish_mutant_sk8_24hpf, biological rep3
Sample type RNA
Source name Zebrafish embryo harvested at 24hpf
Organism Danio rerio
Characteristics Zebrafish mutant whole-embryo RNA
Treatment protocol Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol According to manufacturer's (Affymetrix) recommendations
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description Gene expression data from mutant embryos at 24 hours post fertilization
Data processing Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
Submission date Sep 17, 2008
Last update date Sep 17, 2008
Contact name Nathan Salomonis
Organization name Cincinnati Children's Hospital
Department Biomedical Informatics
Lab Nathan Salomonis
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL1319
Series (1)
GSE12826 Zebrafish 24hpf mutant sk8 gene expression

Data table header descriptions
VALUE GC-RMA calculated Signal intensity

Data table
AFFX-BioB-3_at 5.593638914
AFFX-BioB-5_at 6.254074122
AFFX-BioB-M_at 6.900946078
AFFX-BioC-3_at 7.954844016
AFFX-BioC-5_at 7.9866079
AFFX-BioDn-3_at 10.13768514
AFFX-BioDn-5_at 9.742550585
AFFX-CreX-3_at 13.10636204
AFFX-CreX-5_at 12.13771355
AFFX-DapX-3_at 8.016408783
AFFX-DapX-5_at 6.742335291
AFFX-DapX-M_at 7.229817355
AFFX-Dr-AB076373-1_at 1.974958622
AFFX-Dr-AF292559-1_at 1.765023862
AFFX-Dr-AF292559-2_s_at 3.997101646
AFFX-Dr-AF292559-3_s_at 2.797411507
AFFX-Dr-AF292559-4_s_at 1.853121966
AFFX-Dr-AF292560-1_s_at 2.71778021
AFFX-Dr-AF298789-1_at 1.902616007
AFFX-Dr-AF323980-1_at 2.287710028

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.

Supplementary file Size Download File type/resource
GSM322070.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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