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Sample GSM3223430 Query DataSets for GSM3223430
Status Public on Apr 18, 2019
Title 3h M-,Z- jumu embryos (replicate 1)
Sample type SRA
 
Source name 3h M-,Z- jumu rep 1
Organism Drosophila melanogaster
Characteristics strain: M-,Z- jumu
tissue: embryo
age: 3h
Extracted molecule polyA RNA
Extraction protocol Total RNAs were isolated from embryos at indicated stages using TRIzol reagent (Invitrogen) and mRNAs were then purified using NEBNext® Poly(A) mRNA Magnetic Isolation Module according to the user manual.
Illumina sequencing libraries were constructed according to the manufacturer’s instructions. The libraries were then sequenced using the Illumina Hiseq2500 V4 and NovaSeq platform
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing FastQC was used to make sure that the sequencing data were high quality
Reads were aligned to the dm6 genome assembly using Tophat2/STAR
Differential expression analysis was carried out by cuffdiff
Genome_build: dm6
 
Submission date Jun 26, 2018
Last update date Apr 18, 2019
Contact name Shunmin He
E-mail(s) hesm@ioz.ac.cn
Phone 010-64807279
Organization name Institute of Zoology, Chinese Academy of Sciences
Street address 1 Beichen West Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
 
Platform ID GPL25244
Series (1)
GSE86795 6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda
Relations
BioSample SAMN09487639

Supplementary data files not provided
Raw data are available in SRA
Processed data are available on Series record

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