|
| Status |
Public on Mar 19, 2019 |
| Title |
HS578T cells - pcDNA 3 with cloned EP300 cDNA - biological sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA isolated from cells in culture
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: pcDNA 3 with cloned EP300 cDNA
tumor: breast cancer
cell line: HS578T
|
| Growth protocol |
Cells were grown in low-glucose DMEM containing 10% fetal calf serum, glutamine, insulin and G418
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy total RNA extraction
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChipTM WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 3.5 µg of ss-cDNA were hybridized for 16 hr at 45°C on GeneChip® Cartridge Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix, P/N 00-0079).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.6 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 28, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Ernesto Yague |
| E-mail(s) |
ernesto.yague@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Division of Cancer
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE116370 |
Transcriptome profile of breast cancer HS-578T cells overexpressing EP300 |
|
