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Sample GSM32358 Query DataSets for GSM32358
Status Public on Oct 26, 2004
Title Skin:hariy, from back, Female (MoSk.FeHB)
Sample type MPSS
Source name Skin:hariy, from back, Female total RNA
Organism Mus musculus
Extracted molecule total RNA
Description Strain: C57BL6
Skin:hariy, from back, Female total RNA (MoSk.FeHB) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Library MoSk.FeHB.sig22
Cell type Skin:hariy, from back, Female
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MoSk.FeHB_sig22.5170F.c-20 10/4/2004 337741 8158 QC Passed
MoSk.FeHB_sig22.5170W.a-20 9/17/2004 412511 10638 QC Passed
MoSk.FeHB_sig22.5170W.b-20 9/16/2004 409310 9671 QC Passed
MoSk.FeHB_sig22.5170F.b-20 9/16/2004 412695 10127 QC Passed
MoSk.FeHB_sig22.5170F.a-20 9/15/2004 390908 10615 QC Passed
Run group:
Total Beads successfully sequenced - 1963165
Processed Signatures - 15628
Submission date Oct 14, 2004
Last update date Dec 14, 2005
Contact name Daixing Zhou
Phone 510-670-9441
Fax 510-670-9302
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
Platform ID GPL1010
Series (1)
GSE1581 MPSS mouse transcriptome analysis project

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global

Total number of rows: 9646

Table truncated, full table size 286 Kbytes.

Supplementary file Size Download File type/resource
GSM32358.raw.txt.gz 205.6 Kb (ftp)(http) TXT

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