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Sample GSM324304 Query DataSets for GSM324304
Status Public on Mar 24, 2009
Title day2_siRNA_neg_rep1
Sample type RNA
 
Source name 3T3-L1 fibroblasts (ATCC, CL-173)
Organism Mus musculus
Characteristics fibroblasts
Treatment protocol 3T3-L1 cells were transfected using the LipofectAMINE RNAimax reagent (Invitrogen) according to the manufacturerÆs protocol. Final concentration of siRNA was 50nM. SMARTpool siRNA and negative control siRNA (siCONTROL non-targeting pooled, Dharmacon) were used. Transfected 3T3-L1 fibroblasts were differentiated into adipocytes by treating confluent cells with 0.5mM isobutylmethylxanthine (IBMX) and 0.25?M dexamethasone for 48hrs and then with insulin (5?g/ml) and 1?M Rosiglitazone for 48hrs. Media was replaced every 2 days. Cells were cultured and harvested on day -2, 0, 1, 2, 3, 4, 5, 6 days in relation to rosiglitazone treatment. Three independent biological replicates were collected.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted directly from the cells using the Qiagen RNeasy kit (Qiagen).
Label biotin
Label protocol cRNA was sybthezised using the Ambion's cRNA kit.
 
Hybridization protocol The standard Illumina protocol was followed
Scan protocol The standard Illumina protocol was followed
Description day2_siRNA_neg_rep1
Data processing rank invariant normalization, background correction
 
Submission date Sep 25, 2008
Last update date Mar 24, 2009
Contact name Vinsensius B Vega
E-mail(s) vegav@gis.a-star.edu.sg
Organization name Genome Institute of Singapore
Street address 60 Biopolis Street
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platform ID GPL6103
Series (1)
GSE12929 PPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes

Data table header descriptions
ID_REF
VALUE normalised signal

Data table
ID_REF VALUE
ILMN_2431248 60.5
ILMN_3160339 1
ILMN_3161159 344.9
ILMN_2758597 30
ILMN_2644469 26702.7
ILMN_2656393 14.5
ILMN_3160634 19.6
ILMN_3161394 36866.7
ILMN_2610322 40.1
ILMN_2741606 76.9
ILMN_1247267 159.9
ILMN_2594811 83.2
ILMN_2689425 -25.7
ILMN_2745565 -2.3
ILMN_1212992 -25.2
ILMN_2629183 15
ILMN_1251092 502.8
ILMN_2715803 2353.1
ILMN_2762509 210.8
ILMN_2496817 579.3

Total number of rows: 24607

Table truncated, full table size 441 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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