|
| Status |
Public on Apr 03, 2009 |
| Title |
siCypB.PRL1 |
| Sample type |
RNA |
| |
|
| Source name |
T47D breast cancer cells - si-CypB - treated with prolactin
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: T47D breast cancer cells stably overexpressing CypB small interfering siRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Qiagen RNeasy Micro Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
standard as recommended by illumina
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
The pHIV-7/Puro vectors (si-Luc to target luciferase gene and si-CypB to target CypB gene) were used to generate stable cell lines. Cells were treated with or without prolactin (PRL, 100ng/ml) for 2 hours.
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 1.5.11). It was vst transformed and rsn normalized.
|
| |
|
| Submission date |
Sep 25, 2008 |
| Last update date |
Apr 02, 2009 |
| Contact name |
Feng Fang |
| E-mail(s) |
f-fang@northwestern.edu
|
| Organization name |
Northwestern University
|
| Department |
Department of Pathology
|
| Lab |
Charles Clevenger
|
| Street address |
303 E Superior St Lurie 4-225
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE15505 |
Expression of cyclophilin B is associated with malignant progression and regulation of genes |
|
