|
| Status |
Public on Oct 10, 2008 |
| Title |
zSP1 |
| Sample type |
RNA |
| |
|
| Source name |
Adult zebrafish kidney
|
| Organism |
Danio rerio |
| Characteristics |
Wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted into lysis buffer directly, and total RNAs were extracted using Absolutely RNA Nanoprep Kit (Stratagene) according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was generated by 2 round of in vitro transcription (IVT) using MessageAmp II aRNA Amplification Kit (Applyied Biosystems).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Lymphoid cells (FS-low, SS-low) from zebrafish kidney were subdivided into the SP and MP population (referred to as "zSP" and "zMP") based on the Hoechst fluorescence intensity.
|
| Data processing |
Expression values were calculated by RMA algorithm using the affy package of Bioconductor (http://www.bioconductor.org/).
|
| |
|
| Submission date |
Sep 29, 2008 |
| Last update date |
Sep 30, 2008 |
| Contact name |
Isao Kobayashi |
| Organization name |
Keio University
|
| Department |
Cell Differentiation
|
| Street address |
35 Shinanoma-chi
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE12955 |
Gene expression profile of zebrafish kidney side population (SP) cells |
|
