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Sample GSM325512 Query DataSets for GSM325512
Status Public on Dec 25, 2008
Title wt_206_GFP_pos2, biological replicate 2
Sample type RNA
 
Source name 206_GFP_pos
Organism Danio rerio
Characteristics muscle cells
wild-type zebrafish embryos, 16 hpf
Growth protocol Zebrafish embryos were incubated at 28C in the E3 soluion
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner.
Description Gene expression data from GFP-positive muscle cells
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Sep 30, 2008
Last update date Nov 15, 2008
Contact name Alena Shkumatava
E-mail(s) shkumata@wi.mit.edu
Organization name Whitehead Institute/MIT
Department Bartel Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL1319
Series (1)
GSE12991 Isolation of single miRNA-expressing cells from zebrafish embryos

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 96.3 P 0.004998
AFFX-BioB-M_at 73.4 P 0.000857
AFFX-BioB-3_at 58.1 P 0.012547
AFFX-BioC-5_at 155.1 P 0.000127
AFFX-BioC-3_at 124.7 P 0.00006
AFFX-BioDn-5_at 417.6 P 0.000044
AFFX-BioDn-3_at 1211.6 P 0.000169
AFFX-CreX-5_at 2290.5 P 0.000044
AFFX-CreX-3_at 3362.1 P 0.000044
AFFX-DapX-5_at 32.9 P 0.003595
AFFX-DapX-M_at 325.2 P 0.000857
AFFX-DapX-3_at 724.2 P 0.000052
AFFX-LysX-5_at 7.6 A 0.275146
AFFX-LysX-M_at 46.4 P 0.011384
AFFX-LysX-3_at 172.9 P 0.000052
AFFX-PheX-5_at 14.3 A 0.089478
AFFX-PheX-M_at 35.9 P 0.00141
AFFX-PheX-3_at 233.6 P 0.00011
AFFX-ThrX-5_at 28.6 A 0.185131
AFFX-ThrX-M_at 71.7 P 0.001102

Total number of rows: 15617

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM325512.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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