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Sample GSM325514 Query DataSets for GSM325514
Status Public on Dec 25, 2008
Title wt_206_GFP_pos3, biological replicate 3
Sample type RNA
 
Source name 206_GFP_pos
Organism Danio rerio
Characteristics muscle cells
wild-type zebrafish embryos, 16 hpf
Growth protocol Zebrafish embryos were incubated at 28C in the E3 soluion
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner.
Description Gene expression data from GFP-positive muscle cells
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Sep 30, 2008
Last update date Nov 15, 2008
Contact name Alena Shkumatava
E-mail(s) shkumata@wi.mit.edu
Organization name Whitehead Institute/MIT
Department Bartel Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL1319
Series (1)
GSE12991 Isolation of single miRNA-expressing cells from zebrafish embryos

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 87.1 P 0.005565
AFFX-BioB-M_at 76.5 P 0.001248
AFFX-BioB-3_at 63.1 P 0.033677
AFFX-BioC-5_at 153.4 P 0.00011
AFFX-BioC-3_at 95.7 P 0.000081
AFFX-BioDn-5_at 428.9 P 0.000044
AFFX-BioDn-3_at 1153.2 P 0.000225
AFFX-CreX-5_at 2325.2 P 0.000044
AFFX-CreX-3_at 4032.9 P 0.000052
AFFX-DapX-5_at 31.9 P 0.003595
AFFX-DapX-M_at 266.7 P 0.000972
AFFX-DapX-3_at 599.3 P 0.000052
AFFX-LysX-5_at 4.5 A 0.411365
AFFX-LysX-M_at 46.8 A 0.067678
AFFX-LysX-3_at 130 P 0.00007
AFFX-PheX-5_at 8.8 A 0.313723
AFFX-PheX-M_at 25.8 M 0.046482
AFFX-PheX-3_at 131.3 P 0.00034
AFFX-ThrX-5_at 24 A 0.139482
AFFX-ThrX-M_at 57.3 P 0.002023

Total number of rows: 15617

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM325514.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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