|
| Status |
Public on Dec 25, 2008 |
| Title |
wt_206_GFP_neg3, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
206_GFP_neg
|
| Organism |
Danio rerio |
| Characteristics |
non-muscle cells
wild-type zebrafish embryos, 16 hpf
|
| Growth protocol |
Zebrafish embryos were incubated at 28C in the E3 soluion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner.
|
| Description |
Gene expression data from GFP-negative cells from the rest of the body
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 30, 2008 |
| Last update date |
Nov 15, 2008 |
| Contact name |
Alena Shkumatava |
| E-mail(s) |
shkumata@wi.mit.edu
|
| Organization name |
Whitehead Institute/MIT
|
| Department |
Bartel Lab
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE12991 |
Isolation of single miRNA-expressing cells from zebrafish embryos |
|
