|
| Status |
Public on Apr 20, 2009 |
| Title |
CD4-Hg CD25- (anti CD3, CD28) HG-U133A |
| Sample type |
RNA |
| |
|
| Source name |
CD4-Hg CD25- (anti CD3, CD28) HG-U133A
|
| Organism |
Homo sapiens |
| Characteristics |
genetic modification:
tissue: Blood
|
| Growth protocol |
anti CD3 plate bound, 1µg/ml, 1µg/µl anti CD28
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000.
|
| Description |
CD4-Hg CD25- (anti CD3, CD28)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Oct 02, 2008 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE13017 |
Early-induced genes of human regulatory CD4+CD25hi Treg and CD4+CD25- Th cells |
|
| Relations |
| Reanalyzed by |
GSE86363 |
