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Sample GSM326134 Query DataSets for GSM326134
Status Public on Apr 20, 2009
Title THU CD4+ CD25- (naive) II A unstimulated HG-U133A
Sample type RNA
 
Source name THU CD4+ CD25- (naive) II A unstimulated HG-U133A
Organism Homo sapiens
Characteristics genetic modification:
tissue: Blood
Growth protocol no stimulation
Extracted molecule total RNA
Extraction protocol Rneasy
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000.
Description THU CD4+ CD25- (naiv) II A
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Oct 02, 2008
Last update date Sep 01, 2016
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL96
Series (1)
GSE13017 Early-induced genes of human regulatory CD4+CD25hi Treg and CD4+CD25- Th cells
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 303.8387 A 0.2674626
1053_at 6.665239 A 0.7959779
117_at 84.59164 A 0.2040221
121_at 1371.327 P 0.01700051
1255_g_at 101.3735 A 0.08940502
1294_at 307.664 P 0.01700051
1316_at 137.093 P 0.01493657
1320_at 18.62346 A 0.7492763
1405_i_at 10.89445 A 0.9210943
1431_at 72.88039 A 0.08133665
1438_at 90.7705 A 0.7654434
1487_at 162.959 A 0.3586904
1494_f_at 285.9835 A 0.06686494
1598_g_at 398.7019 A 0.162935
160020_at 659.9573 P 0.04899465
1729_at 473.8075 P 0.02785966
177_at 71.82411 A 0.3395579
1773_at 27.36561 A 0.5819307
179_at 509.1561 A 0.2189833
1861_at 114.8999 A 0.2847472

Total number of rows: 22283

Table truncated, full table size 675 Kbytes.




Supplementary file Size Download File type/resource
GSM326134.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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