|
| Status |
Public on Jul 10, 2018 |
| Title |
Glutamatergic neuron_1210B2 |
| Sample type |
RNA |
| |
|
| Source name |
Glutamatergic neuron induced from iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Glutamatergic neuron
iPSc clone: 1210B2
diagnosis: healthy
|
| Growth protocol |
neurons were induced from iPSCs and cultured in neuron culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from induced neuron by Rneasy kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip™ WT PLUS Reagent Kit)
|
| |
|
| Hybridization protocol |
Fragmented and labeled ss-cDNA were hybridized for 16 hr at 45℃ on GeneChip Clariom S array, human. GeneChips were washed and stained in the Genechip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Gene chip Scanner 3000 7G (Thermo Fisher Scientific)
|
| Data processing |
Data were processed using the Transcriptome Analysis Console (TAC) 4.0 Software (Thermo Fisher Scientific).
|
| |
|
| Submission date |
Jul 09, 2018 |
| Last update date |
Sep 06, 2019 |
| Contact name |
Takaya Ishii |
| Organization name |
Keio university
|
| Department |
School of medicine
|
| Lab |
Physiology
|
| Street address |
Shinanomachi35
|
| City |
shinjuku-ku, Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE116820 |
Expression data of Glutarmatergic neuron and GABAergic neruon induced from iPSCs |
|
