|
Status |
Public on Jul 10, 2018 |
Title |
GABAergic neuron_BP1-1 |
Sample type |
RNA |
|
|
Source name |
GABAergic neuron induced from iPSC
|
Organism |
Homo sapiens |
Characteristics |
cell type: GABAergic neuron iPSc clone: BP001-1 diagnosis: Bipolar disorder
|
Growth protocol |
neurons were induced from iPSCs and cultured in neuron culture medium
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from induced neuron by Rneasy kit (QIAGEN)
|
Label |
biotin
|
Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip™ WT PLUS Reagent Kit)
|
|
|
Hybridization protocol |
Fragmented and labeled ss-cDNA were hybridized for 16 hr at 45℃ on GeneChip Clariom S array, human. GeneChips were washed and stained in the Genechip Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Gene chip Scanner 3000 7G (Thermo Fisher Scientific)
|
Data processing |
Data were processed using the Transcriptome Analysis Console (TAC) 4.0 Software (Thermo Fisher Scientific).
|
|
|
Submission date |
Jul 09, 2018 |
Last update date |
Sep 06, 2019 |
Contact name |
Takaya Ishii |
Organization name |
Keio university
|
Department |
School of medicine
|
Lab |
Physiology
|
Street address |
Shinanomachi35
|
City |
shinjuku-ku, Tokyo |
ZIP/Postal code |
160-8582 |
Country |
Japan |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE116820 |
Expression data of Glutarmatergic neuron and GABAergic neruon induced from iPSCs |
|