|
Status |
Public on Jul 13, 2018 |
Title |
Healthy donor 3 CD40L/IL21 |
Sample type |
RNA |
|
|
Source name |
transitional B cells from normal donors
|
Organism |
Homo sapiens |
Characteristics |
cell type: transitional B cells diagnosis: healthy/normal
|
Treatment protocol |
B cells were stimulated with recombinant CD40 ligand alone or together with IL-21 (50 ng/mL)
|
Growth protocol |
Human transitional B cells were cultured in RPMI1640 containing 10% FCS for 4 days
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
1 ng of total RNA was used as input for the amplification and labelling of samples using the Affymetrix WT Pico Kit
|
|
|
Hybridization protocol |
3 µg fragmented, biotin labelled ss-cDNA was hybridised to Affymetrix Human Gene Array version 2.1ST. Samples were hybridised to arrays for 16 hours at 45°C in the Affymetrix GeneTitan Instrument
|
Scan protocol |
Arrays were washed and scanned on the Affymetrix GeneTitanand Affymetrix GC3000 scanner instrument as per manufacturers protocol
|
Description |
RNA from normal transitional B cells stimulated with CD40L + IL-21
|
Data processing |
Bioconductor packages implemented in the R statistical computing environment, version 3.4.1. The file normalized_data_with_all_control_probesets.txt contains the log2 RMA data for all probe sets including control probe sets. The sample data tables include a subset of the control probe sets found on platform GPL23159.
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|
|
Submission date |
Jul 12, 2018 |
Last update date |
Jul 13, 2018 |
Contact name |
Stuart Tangye |
E-mail(s) |
s.tangye@garvan.org.au
|
Phone |
+61 2 9295 8455
|
Organization name |
Garvan Institute of Medical Research
|
Department |
Immunology
|
Street address |
384 Victoria St
|
City |
Darlinghurst |
State/province |
NSW |
ZIP/Postal code |
2010 |
Country |
Australia |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE116999 |
Identification of differentially expressed genes between control and PIK3CD GOF human activated transitional B cells |
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