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Sample GSM327236 Query DataSets for GSM327236
Status Public on Oct 08, 2008
Title control retina T6-sample3
Sample type RNA
 
Source name lesioned retina 6hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 6hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
 
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.423427582
AFFX-BioB-5_at 8.309533119
AFFX-BioB-M_at 8.984965324
AFFX-BioC-3_at 8.831907272
AFFX-BioC-5_at 9.466686249
AFFX-BioDn-3_at 11.06509686
AFFX-BioDn-5_at 10.35631084
AFFX-CreX-3_at 13.12265015
AFFX-CreX-5_at 12.61106873
AFFX-DapX-3_at 2.103431463
AFFX-DapX-5_at 2.155514956
AFFX-DapX-M_at 2.024867773
AFFX-Dr-AB076373-1_at 2.091419935
AFFX-Dr-acta1-3_at 11.00395679
AFFX-Dr-acta1-5_at 5.834430695
AFFX-Dr-acta1-5_x_at 5.451445103
AFFX-Dr-acta1-M_at 7.237398624
AFFX-Dr-AF292559-1_at 2.320758581
AFFX-Dr-AF292559-2_s_at 1.747138739
AFFX-Dr-AF292559-3_s_at 1.876211882

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM327236.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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