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Sample GSM327240 Query DataSets for GSM327240
Status Public on Oct 08, 2008
Title control retina T12-sample4
Sample type RNA
Source name lesioned retina 12hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 12hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
VALUE RMA-calculated Signal intensity

Data table
AFFX-BioB-3_at 9.127137184
AFFX-BioB-5_at 8.684627533
AFFX-BioB-M_at 9.479125977
AFFX-BioC-3_at 9.454054832
AFFX-BioC-5_at 10.09852791
AFFX-BioDn-3_at 11.65319252
AFFX-BioDn-5_at 10.94019508
AFFX-CreX-3_at 13.64739799
AFFX-CreX-5_at 13.10757923
AFFX-DapX-3_at 2.1354146
AFFX-DapX-5_at 2.17416048
AFFX-DapX-M_at 2.045898676
AFFX-Dr-AB076373-1_at 2.334196568
AFFX-Dr-acta1-3_at 6.622968674
AFFX-Dr-acta1-5_at 3.826790094
AFFX-Dr-acta1-5_x_at 4.8476758
AFFX-Dr-acta1-M_at 2.694972515
AFFX-Dr-AF292559-1_at 2.285573721
AFFX-Dr-AF292559-2_s_at 1.899861455
AFFX-Dr-AF292559-3_s_at 2.013696432

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.

Supplementary file Size Download File type/resource
GSM327240.CEL.gz 1.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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