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Sample GSM327243 Query DataSets for GSM327243
Status Public on Oct 08, 2008
Title control retina T24-sample4
Sample type RNA
 
Source name lesioned retina 24hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 24hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
 
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.749365807
AFFX-BioB-5_at 8.652682304
AFFX-BioB-M_at 9.20583725
AFFX-BioC-3_at 9.26171875
AFFX-BioC-5_at 9.849829674
AFFX-BioDn-3_at 11.64200497
AFFX-BioDn-5_at 10.78945637
AFFX-CreX-3_at 13.3856678
AFFX-CreX-5_at 12.99240208
AFFX-DapX-3_at 2.057863474
AFFX-DapX-5_at 2.194961071
AFFX-DapX-M_at 2.08231163
AFFX-Dr-AB076373-1_at 2.407841682
AFFX-Dr-acta1-3_at 7.784096241
AFFX-Dr-acta1-5_at 4.428962231
AFFX-Dr-acta1-5_x_at 4.617960453
AFFX-Dr-acta1-M_at 3.185314178
AFFX-Dr-AF292559-1_at 2.279733419
AFFX-Dr-AF292559-2_s_at 1.745426893
AFFX-Dr-AF292559-3_s_at 1.891992569

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM327243.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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