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Sample GSM327244 Query DataSets for GSM327244
Status Public on Oct 08, 2008
Title control retina T48-sample2
Sample type RNA
 
Source name lesioned retina 48hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 48hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
 
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.637664795
AFFX-BioB-5_at 8.264036179
AFFX-BioB-M_at 8.942100525
AFFX-BioC-3_at 8.838670731
AFFX-BioC-5_at 9.443249702
AFFX-BioDn-3_at 11.35939789
AFFX-BioDn-5_at 10.39057922
AFFX-CreX-3_at 13.31343174
AFFX-CreX-5_at 12.65703773
AFFX-DapX-3_at 2.30568409
AFFX-DapX-5_at 2.523316383
AFFX-DapX-M_at 2.314036131
AFFX-Dr-AB076373-1_at 2.430258274
AFFX-Dr-acta1-3_at 5.918151379
AFFX-Dr-acta1-5_at 4.945940495
AFFX-Dr-acta1-5_x_at 4.717490673
AFFX-Dr-acta1-M_at 2.492552996
AFFX-Dr-AF292559-1_at 2.100800753
AFFX-Dr-AF292559-2_s_at 1.832042336
AFFX-Dr-AF292559-3_s_at 1.974487901

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM327244.CEL.gz 1.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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