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Sample GSM327245 Query DataSets for GSM327245
Status Public on Oct 08, 2008
Title control retina T48-sample3
Sample type RNA
 
Source name lesioned retina 48hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 48hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
 
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 9.024345398
AFFX-BioB-5_at 9.003938675
AFFX-BioB-M_at 9.561779976
AFFX-BioC-3_at 9.286409378
AFFX-BioC-5_at 10.00991249
AFFX-BioDn-3_at 11.57181931
AFFX-BioDn-5_at 10.71018887
AFFX-CreX-3_at 13.27828503
AFFX-CreX-5_at 12.88644505
AFFX-DapX-3_at 2.10873723
AFFX-DapX-5_at 2.131385326
AFFX-DapX-M_at 2.067508459
AFFX-Dr-AB076373-1_at 2.546093702
AFFX-Dr-acta1-3_at 3.737546921
AFFX-Dr-acta1-5_at 5.342693806
AFFX-Dr-acta1-5_x_at 5.229120255
AFFX-Dr-acta1-M_at 2.043021917
AFFX-Dr-AF292559-1_at 2.220455647
AFFX-Dr-AF292559-2_s_at 1.882014751
AFFX-Dr-AF292559-3_s_at 1.875116229

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM327245.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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