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Sample GSM327246 Query DataSets for GSM327246
Status Public on Oct 08, 2008
Title control retina T48-sample4
Sample type RNA
 
Source name lesioned retina 48hrs after light onset
Organism Danio rerio
Characteristics wild type, adult Danio retina
Extracted molecule total RNA
Extraction protocol Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
Label biotin
Label protocol mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
 
Hybridization protocol Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene Expression from lesioned retina 48hrs after light onset.
Data processing The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
 
Submission date Oct 06, 2008
Last update date Oct 07, 2008
Contact name Peter F. Hitchcock
E-mail(s) peterh@umich.edu
Phone 734-763-8169
Fax 734-936-7231
Organization name University of Michigan
Department Ophthy and Vis Sci
Street address 1000 Wall Street
City Ann Arbor
State/province MI
ZIP/Postal code 48104
Country USA
 
Platform ID GPL1319
Series (1)
GSE13068 Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.651244164
AFFX-BioB-5_at 8.512710571
AFFX-BioB-M_at 9.179800987
AFFX-BioC-3_at 8.993645668
AFFX-BioC-5_at 9.661705017
AFFX-BioDn-3_at 11.35243225
AFFX-BioDn-5_at 10.5163517
AFFX-CreX-3_at 13.17615128
AFFX-CreX-5_at 12.79583836
AFFX-DapX-3_at 2.317187786
AFFX-DapX-5_at 2.182309151
AFFX-DapX-M_at 2.171792269
AFFX-Dr-AB076373-1_at 2.486497402
AFFX-Dr-acta1-3_at 3.523305655
AFFX-Dr-acta1-5_at 5.369116306
AFFX-Dr-acta1-5_x_at 4.898218632
AFFX-Dr-acta1-M_at 2.303344011
AFFX-Dr-AF292559-1_at 2.33780551
AFFX-Dr-AF292559-2_s_at 1.779145122
AFFX-Dr-AF292559-3_s_at 1.869881988

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM327246.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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