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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 01, 2008 |
| Title |
hES_H3K4me3_ChIPSeq |
| Sample type |
SRA |
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| Source name |
Chromatin IP against H3K4me3
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| Organism |
Homo sapiens |
| Characteristics |
Embyonic stem cells
line: H9 (passage 45)
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| Growth protocol |
Embryonic stem cells were cultured as described in (Mikkelsen et al. 2007, PMID 17603471) and (Thomson et al. 1998, PMID 9804556 )
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
All ChIP experiments were performed as described in (Mikkelsen et al. 2007, PMID 17603471), except for Suz12 and Ezh2, for which an additional nucleus isolation step was included, as described in (Weinmann et al. 2001, PMID 11564866).
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
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| Description |
Chromatin IP against H3K4me3
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| Data processing |
Primary sequence data were filtered and aligned as described in (Mikkelsen et al. 2007, PMID 17603471).
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| Submission date |
Oct 07, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Tarjei S Mikkelsen |
| Organization name |
Broad Institute
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| Street address |
7 Cambridge Center
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platform ID |
GPL9052 |
| Series (1) |
| GSE13084 |
Mapping polycomb complexes in human and mouse embryonic stem cells |
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| Relations |
| SRA |
SRX003845 |
| BioSample |
SAMN02195723 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM327662_hES.H3K4me3.aligned.txt.gz |
191.0 Mb |
(ftp)(http) |
TXT |
| GSM327662_hES.H3K4me3.densities.txt.gz |
314.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available on Series record |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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