|
| Status |
Public on Oct 10, 2008 |
| Title |
Dap12-KO untreated |
| Sample type |
RNA |
| |
|
| Source name |
Dap12-KO untreated
|
| Organism |
Mus musculus |
| Characteristics |
Mice used in this study were on a C57BL/6J background, 10-30 weeks old and housed at specific-pathogen free conditions.
Peritoneal macrophages from DAP12 KO
|
| Treatment protocol |
Cells were treated for 18 hours on permanox slides.
|
| Growth protocol |
Cells were harvested from the peritoneal cavity of thioglycolate treated mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated antisense cRNA was prepared by using two cycles of in vitro amplification according to the Affymetrix Small Sample Labeling Protocol II.
|
| |
|
| Hybridization protocol |
15µg of biotinylated cRNA was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression of resting macrophages
|
| Data processing |
All data analyses were performed by using Bioconductor version 1.5 for the statistical software R. RMA was used as measurement
|
| |
|
| Submission date |
Oct 09, 2008 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Laura Helming |
| E-mail(s) |
Laura.Helming@lrz.tu-muenchen.de
|
| Phone |
0049 (0)89 4140 7454/6884
|
| Fax |
0049 (0)89 4140 7454/6884
|
| URL |
http://www.uni-koeln.de/med-fak/immh/
|
| Organization name |
Institut für medizinische Mikrobiologie, Immunologie und Hygiene
|
| Street address |
Trogerstr. 30
|
| City |
Munchen |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE13140 |
Basal and IL-4 response in DAP12 (TYROBP) KO mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
