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Status |
Public on Dec 07, 2008 |
Title |
Fibroblast, technical rep3 |
Sample type |
RNA |
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Source name |
Adult somatic skin fibroblasts(0235#)
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Organism |
Macaca mulatta |
Characteristics |
3 years old Rhesus monkey(0235#) ear skin firoblasts
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Treatment protocol |
ES and ES cells with good morphology were manually removed from culture for total RNA purification.
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Growth protocol |
Fibroblasts were cultured in Dulbeccos modified eagle medium (DMEM, Hyclone) containing 10% fetal bovine serum (FBS, Invitrogen). Embryonic stem (ES) cells (R366.4) were maintained on mitomycin C-treated MEFs in DMEM (Invitrogen) with 15% FBS (Hyclone),
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rhesus Macaque Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
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Description |
Gene expression data from Rhesus monkey(0235#) ear skin firoblasts.
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Data processing |
Raw data were first normalized with dChip. To compare every two samples, the median of each probe in three replications of each sample was chosen as the signal value of this probe by the following selection criteria: coefficient of variation (C.V) <0.5 i
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Submission date |
Oct 10, 2008 |
Last update date |
Dec 06, 2008 |
Contact name |
hongkui deng |
E-mail(s) |
hongkui_deng@pku.edu.cn
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Phone |
86-10-6275-6474
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Fax |
86-10-6275-6474
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Organization name |
Peking University
|
Department |
College of Life Sciences
|
Street address |
Yiheyuan Road 5#, Haidian District
|
City |
Beijing |
ZIP/Postal code |
100871 |
Country |
China |
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Platform ID |
GPL3535 |
Series (1) |
GSE13146 |
Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts |
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