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Sample GSM329250 Query DataSets for GSM329250
Status Public on Dec 07, 2008
Title Fibroblast, technical rep3
Sample type RNA
 
Source name Adult somatic skin fibroblasts(0235#)
Organism Macaca mulatta
Characteristics 3 years old Rhesus monkey(0235#) ear skin firoblasts
Treatment protocol ES and ES cells with good morphology were manually removed from culture for total RNA purification.
Growth protocol Fibroblasts were cultured in Dulbecco’s modified eagle medium (DMEM, Hyclone) containing 10% fetal bovine serum (FBS, Invitrogen). Embryonic stem (ES) cells (R366.4) were maintained on mitomycin C-treated MEFs in DMEM (Invitrogen) with 15% FBS (Hyclone),
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rhesus Macaque Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from Rhesus monkey(0235#) ear skin firoblasts.
Data processing Raw data were first normalized with dChip. To compare every two samples, the median of each probe in three replications of each sample was chosen as the signal value of this probe by the following selection criteria: coefficient of variation (C.V) <0.5 i
 
Submission date Oct 10, 2008
Last update date Dec 06, 2008
Contact name hongkui deng
E-mail(s) hongkui_deng@pku.edu.cn
Phone 86-10-6275-6474
Fax 86-10-6275-6474
Organization name Peking University
Department College of Life Sciences
Street address Yiheyuan Road 5#, Haidian District
City Beijing
ZIP/Postal code 100871
Country China
 
Platform ID GPL3535
Series (1)
GSE13146 Generation of Induced Pluripotent Stem Cells from Adult Rhesus Monkey Fibroblasts

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 281.92 P
AFFX-BioB-M_at 386.26 P
AFFX-BioB-3_at 256.83 P
AFFX-BioC-5_at 598.10 P
AFFX-BioC-3_at 688.30 P
AFFX-BioDn-5_at 1134.86 P
AFFX-BioDn-3_at 1873.89 P
AFFX-CreX-5_at 5419.91 P
AFFX-CreX-3_at 5139.35 P
AFFX-DapX-5_at 314.57 P
AFFX-DapX-M_at 767.35 P
AFFX-DapX-3_at 938.31 P
AFFX-LysX-5_at 37.36 P
AFFX-LysX-M_at 93.91 P
AFFX-LysX-3_at 156.32 P
AFFX-PheX-5_at 35.24 P
AFFX-PheX-M_at 123.95 P
AFFX-PheX-3_at 101.16 P
AFFX-ThrX-5_at 78.92 P
AFFX-ThrX-M_at 118.74 P

Total number of rows: 52865

Table truncated, full table size 1527 Kbytes.




Supplementary file Size Download File type/resource
GSM329250.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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