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Sample GSM329384 Query DataSets for GSM329384
Status Public on Oct 16, 2008
Title Embryos injected with 15uM coMO biological repl. 2
Sample type RNA
 
Source name 72hpf zebrafish embryos
Organism Danio rerio
Characteristics wild type
control morpholinos
Treatment protocol When embryo reached 72hpf they were euthanized in MS222 and put in RNAlater solution at 4°C overnight. The samples were stored until RNA isolation at -20°C
Growth protocol Eggs were pooled from different aquaria, washed with a E3 medium and injected with ERbeta2 MO or coMO at 15uM until the 4 cell stage. After they were raised in petri dishes until 72hpf at 28°C.
Extracted molecule total RNA
Extraction protocol RNA isolation was performed with Rneasy Mini Kit, Qiagen, according to the manufacturer's instructions.
Label biotin
Label protocol biotin-labeling using a IVT Labeling Kit (Affymetrix Inc.)
 
Hybridization protocol Affymetrix Gene Expression protocol using Fragmentation Buffer (Affymetrix Inc., P/N 900371), Hybridization Mix (Affymetrix Inc., P/N 900720), Hybridization Controls and Control Oligonucleotide B2 (Affymetrix Inc., P/N 900454). Arrays were washed using an Affymetrix Fluidics Station 450 (FS450_0004 protocol)
Scan protocol Affymetrix GeneChip Scanner 3000 (Affymetrix Inc.), according to manufacturer
Description gene expression in 72hpf zebrafish embryos
Data processing Raw data processing was performed using the Affymetrix AGCC software. After hybridization and scanning, probe cell intensities were calculated by means of the MAS5 algorithm. The data were then analyzed with Genespring. The data were normalized per chip (50th percentil) and per gene (to median). They were finally filtered by fold change (fold change data files are on the Series record).
 
Submission date Oct 10, 2008
Last update date Oct 15, 2008
Contact name Mirjam Föhlicher
E-mail(s) mirjam.froehlicher@eawag.ch
Organization name eawag
Street address überlandstrasse 133
City Dübendorf
ZIP/Postal code 8600
Country Switzerland
 
Platform ID GPL1319
Series (2)
GSE13157 The role of ERbeta2 in zebrafish neuromasts development 15uM
GSE13771 The role of ERbeta2 in zebrafish neuromasts development

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 363.22 P 0.000971263
AFFX-BioB-M_at 512.305 P 4.42873e-05
AFFX-BioB-3_at 322.075 P 0.000662269
AFFX-BioC-5_at 1033.2 P 7.00668e-05
AFFX-BioC-3_at 950.723 P 4.42873e-05
AFFX-BioDn-5_at 2077.68 P 4.42873e-05
AFFX-BioDn-3_at 4307.32 P 5.16732e-05
AFFX-CreX-5_at 10436.1 P 4.42873e-05
AFFX-CreX-3_at 20531.4 P 4.42873e-05
AFFX-DapX-5_at 563.066 P 0.000169227
AFFX-DapX-M_at 953.855 P 9.4506e-05
AFFX-DapX-3_at 1456.84 P 4.42873e-05
AFFX-LysX-5_at 50.9385 P 0.00618711
AFFX-LysX-M_at 156.387 P 0.0219019
AFFX-LysX-3_at 214.848 P 0.000169227
AFFX-PheX-5_at 92.8629 P 0.00401721
AFFX-PheX-M_at 114.402 P 0.00141043
AFFX-PheX-3_at 304.187 P 0.000340305
AFFX-ThrX-5_at 151.881 P 0.00227496
AFFX-ThrX-M_at 192.27 P 0.000126798

Total number of rows: 15617

Table truncated, full table size 573 Kbytes.




Supplementary file Size Download File type/resource
GSM329384.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM329384.CHP.gz 172.5 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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