|
Status |
Public on Dec 13, 2018 |
Title |
αGFP_4 [9_S9] |
Sample type |
SRA |
|
|
Source name |
αGFP
|
Organism |
Homo sapiens |
Characteristics |
tissue: pancreas cell type: alpha-cells cultured or grafted: 1 week transduction: GFP
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy Micro kit, and treated with RNase-free DNaseI (Qiagen) Extracted RNA sample was assessed for the quality by Agilent bioanalyzer prior to library generation. Reverse transcription and cDNA amplification were performed using the SMARTer Ultra Low RNA kit (Clontech). cDNA library was prepared using Nextera XT DNA Sample Preparation kit (Illumina), and multiplexed.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
9_S9
|
Data processing |
The sequencing quality control was done with FASTQC v.0.11.2, followed by sequence alignment to the human reference genome (hg38) using the TopHat v2.0.13 (default parameters). Biological quality control and summarization were done with the PicardTools v1.80. Genome_build: hg38 Supplementary_files_format_and_content: tab-delimited text files include raw counts of sequencing reads for each sample
|
|
|
Submission date |
Jul 20, 2018 |
Last update date |
Dec 14, 2018 |
Contact name |
Kenichiro Furuyama |
E-mail(s) |
kenichiro.furuyama@unige.ch
|
Phone |
+41 22 379 5223
|
Organization name |
University of Geneva
|
Department |
Genetic Medicine and Development
|
Lab |
Pedro Herrera
|
Street address |
1, rue Michel-Servet
|
City |
Geneva |
State/province |
Geneva |
ZIP/Postal code |
CH1211 |
Country |
Switzerland |
|
|
Platform ID |
GPL20301 |
Series (1) |
GSE117454 |
Diabetes Remission Using Glucose-Responsive Insulin-Producing Human α-Cells |
|
Relations |
BioSample |
SAMN09698353 |
SRA |
SRX4414170 |