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Sample GSM3295689 Query DataSets for GSM3295689
Status Public on Dec 13, 2018
Title αPM_1 [12_S12]
Sample type SRA
 
Source name αPM
Organism Homo sapiens
Characteristics tissue: pancreas
cell type: alpha-cells
cultured or grafted: 1 week
transduction: PM
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNeasy Micro kit, and treated with RNase-free DNaseI (Qiagen)
Extracted RNA sample was assessed for the quality by Agilent bioanalyzer prior to library generation. Reverse transcription and cDNA amplification were performed using the SMARTer Ultra Low RNA kit (Clontech). cDNA library was prepared using Nextera XT DNA Sample Preparation kit (Illumina), and multiplexed.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description 12_S12
Data processing The sequencing quality control was done with FASTQC v.0.11.2, followed by sequence alignment to the human reference genome (hg38) using the TopHat v2.0.13 (default parameters).
Biological quality control and summarization were done with the PicardTools v1.80.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include raw counts of sequencing reads for each sample
 
Submission date Jul 20, 2018
Last update date Dec 14, 2018
Contact name Kenichiro Furuyama
E-mail(s) kenichiro.furuyama@unige.ch
Phone +41 22 379 5223
Organization name University of Geneva
Department Genetic Medicine and Development
Lab Pedro Herrera
Street address 1, rue Michel-Servet
City Geneva
State/province Geneva
ZIP/Postal code CH1211
Country Switzerland
 
Platform ID GPL20301
Series (1)
GSE117454 Diabetes Remission Using Glucose-Responsive Insulin-Producing Human α-Cells
Relations
BioSample SAMN09698350
SRA SRX4414173

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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