|
| Status |
Public on Oct 01, 2018 |
| Title |
Raw264_Gpr137B-KO_IL4minus_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Raw264 cells_Gpr137B-KO_IL4minus
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Raw264
cell type: Mouse macrophage cell line
genotype/variation: Gpr137b -/-
|
| Treatment protocol |
Raw264 cells were treated with mouse IL-4 to induce M2 macrophage polarization.
|
| Growth protocol |
Raw264 cells were cultured in Eagle's MEM supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Raw264 cells using RNAiso (Takara-bio, Kusatsu, Japan) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The total RNA was subjected to cRNA synthesis followed by ssDNA synthesis using GeneChip WT Plus Reagent Kit (Affymetrix). The ssDNA (5.5 micrograms) was then fragmentated and biotin-labeled using GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer's manual.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized for 17 hr on Clariom S Array (mouse) using GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
Clone#7-3-4_IL4minus
|
| Data processing |
The data were analyzed with Affymetrix® GeneChip Command Console using SST-RMA as a summarization algorithm.
|
| |
|
| Submission date |
Jul 24, 2018 |
| Last update date |
Oct 01, 2018 |
| Contact name |
OSAMU ISHIBASHI |
| E-mail(s) |
ishibashi@biochem.osakafu-u.ac.jp
|
| Phone |
+81-72-254-9474
|
| Organization name |
Osaka Prefecture University
|
| Department |
Grad Sch Life & Environmental Sciences
|
| Lab |
Biological Macromolecules
|
| Street address |
1-1 Gakuen-cho, Naka-ku
|
| City |
Sakai |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE117578 |
Array-based genome-wide transcriptome analysis of IL-4-treated RAW264 macrohage cell line |
|
