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| Status |
Public on Feb 11, 2019 |
| Title |
NOD_14w_A |
| Sample type |
SRA |
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| Source name |
Pancreatic islets
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| Organism |
Mus musculus |
| Characteristics |
age: 14 weeks
strain: NOD/ShiltJ
tissue: Pancreas
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| Extracted molecule |
total RNA |
| Extraction protocol |
islets were extracted, single cell suspension was made and CD45 cells were depleted.
scRNA libraries were prepared for sequencing using standard 10X Genomics protocol
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
NOD_14w_A
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| Data processing |
Cell Ranger v2.0.0
Genome_build: mm10
Supplementary_files_format_and_content: filtered gene barcode matrices from cellranger output: genes.tsv, barcodes.tsv, matrix.mtx (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/2.0/output/overview)
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| Submission date |
Jul 27, 2018 |
| Last update date |
Feb 13, 2019 |
| Contact name |
Anil Bhushan |
| Organization name |
UCSF
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| Department |
Diabetes Center
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| Lab |
Bhushan Lab
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| Street address |
513 Parnassus ave
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE117770 |
Targeted elimination of senescent beta cells prevents Type 1 Diabetes |
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| Relations |
| BioSample |
SAMN09726650 |
| SRA |
SRX4475141 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM3308548_NOD_14w_A_barcodes.tsv.gz |
20.5 Kb |
(ftp)(http) |
TSV |
| GSM3308548_NOD_14w_A_genes.tsv.gz |
212.7 Kb |
(ftp)(http) |
TSV |
| GSM3308548_NOD_14w_A_matrix.mtx.gz |
36.4 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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