|
| Status |
Public on Aug 02, 2018 |
| Title |
Sulfisoxazole (SFX) treated MDA-MB231 (24 h treatment)_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Sulfisoxazole (SFX) treated MDA-MB231 (24 h treatment)_rep2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB231
tissue: mammary gland/breast
cell type: epithelial
disease: adenocarcinoma/derived from metastatic site
|
| Treatment protocol |
Cells were treated 100μM of SFX for 24 hours under serum-free condition.
|
| Growth protocol |
SKMEL28 cells were grown under following condition: Eagle's Minimum Essential Medium (EMEM) with 10 % fetal bovine serum (FBS) and 1% antibiotic and antimycotic agent. MDA-MB231 cells were grown under following condition: Dulbecco Modified Eagle Medium (DMEM) with 10 % fetal bovine serum (FBS) and 1% antibiotic and antimycotic agent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
5.5μg of labeled DNA target was hybridized to the Affmetrix GeneChip Clariom S Human Array at 45 °C for 16hours. Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450
|
| Scan protocol |
Hybridized arrays were scanned on a GCS3000 Scanner (Affymetrix)
|
| Description |
Gene expression data from DMSO sulfisoxazole-treated MDA-MB231
|
| Data processing |
The data were summarized and normalized with robust multi-average (RMA) method implemented in Affymetrix Power Tools (APT).
|
| |
|
| Submission date |
Aug 01, 2018 |
| Last update date |
Aug 02, 2018 |
| Contact name |
Moon-Chang Baek |
| E-mail(s) |
mcbaek@knu.ac.kr
|
| Phone |
82-10-9357-0794
|
| Organization name |
Kyungpook National University
|
| Street address |
Gukchebosang-ro 680
|
| City |
Daegu |
| ZIP/Postal code |
41944 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE117991 |
Expression data from sulfisoxazole-treated cancer cells |
|
