|
| Status |
Public on Nov 23, 2018 |
| Title |
polyRNA_IFNg+silves_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
polysomal RNA, A375, IFNg 24h, silves, 2h
|
| Organism |
Homo sapiens |
| Characteristics |
tumor type: melanoma cells
cell line: A375
fraction: polysomal associated RNA
time point: 24h
agent: Interferon-gamma and 2h silvestrol
|
| Treatment protocol |
One day after spliting A375 cells were treated for 2 hours with DMSO or silvestrol, 24 hourswith Interferon-gamma
|
| Growth protocol |
A375 melanoma cells were cultured according to the ATCC protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and polysomal associated RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Accoding to Affymetrix protocol
|
| |
|
| Hybridization protocol |
Accoding to Affymetrix protocol
|
| Scan protocol |
Accoding to Affymetrix protocol
|
| Description |
Gene expression data from polysomal associated RNA from A375 treated 24h with Interferon-gamma and 2h with silvestrol
|
| Data processing |
The data were analyzed with Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Aug 14, 2018 |
| Last update date |
Nov 23, 2018 |
| Contact name |
Caroline Robert |
| Organization name |
Institut Gustave Roussy
|
| Street address |
114 rue Edouard Vaillant, Bâtiment de Médecine Moléculaire
|
| City |
Villejuif |
| State/province |
ILE-DE-FRANCE |
| ZIP/Postal code |
94805 |
| Country |
France |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE118521 |
Expression data from A375 melanoma cells treated with Interferon-gamma and silvestrol |
|
