|
| Status |
Public on Oct 01, 2009 |
| Title |
p4_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic stem cells, fraction p4
|
| Organism |
Homo sapiens |
| Characteristics |
HES2 standard culture
|
| Treatment protocol |
HES2 cells were subjected to FACs sorting, and fractions p4, p5, p6, and p7 were collected.
|
| Growth protocol |
HES2 ESCs were grown in standard ES culture conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were prepared using Qiagen RNeasy Micro Kit (Qiagen) and then assayed using the Agilent Systems Bioanalyzer 2100 to ensure that high-quality RNA was used for the array experiments.
|
| Label |
Cy3
|
| Label protocol |
Targets were prepared from 100 ng total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791).
|
| |
|
| Hybridization protocol |
Illumina Human 6 BeadChips were hybridized, washed, stained and scanned according to Illumina's protocol.
|
| Scan protocol |
Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
|
| Description |
Replicate 2 of 3
1705168044_E
|
| Data processing |
Data were first extracted with Illumina BeadStudio software without normalisation. Data was then uploaded into R, where RMA background adjustment and Quantile normalisation was performed using the BeadExplorer package.
|
| |
|
| Submission date |
Oct 14, 2008 |
| Last update date |
Oct 01, 2009 |
| Contact name |
Sean Grimmond |
| E-mail(s) |
s.grimmond@imb.uq.edu.au
|
| Organization name |
University of Queensland
|
| Department |
Institute for Molecular Bioscience
|
| Lab |
Expression Genomics Lab
|
| Street address |
Services Rd
|
| City |
St Lucia |
| State/province |
Q |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE13201 |
HES2 embryonic stem cells (ESCs) grown in standard conditions and FACs sorted for GCTM-2 and CD9 |
|
