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Sample GSM333645 Query DataSets for GSM333645
Status Public on Jan 15, 2009
Title iPS OSE #T8, biological replicate 2
Sample type RNA
 
Source name mouse induced pluripotent cells (iPS)
Organism Mus musculus
Characteristics OSE-induced iPS
Treatment protocol n.a.
Growth protocol Mouse iPS cells were cultured at 37°C with 5% CO2. Mouse iPS cells were maintained on mitomycin C-treated MEF feeders in Dulbecco’s modified Eagle medium (DMEM; GIBCO), supplemented with 15% heat-inactivated fetal bovine serum (FBS; GIBCO), 0.055 mM Beta-mercaptoethanol (GIBCO), 2 mM L-glutamine, 0.1 mM MEM nonessential amino acid, 5,000 units/ml penicillin/streptomycin and 1,000 units/ml of LIF (Chemicon).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and purified with RNeasy minikit (Qiagen).
Label Biotin
Label protocol Biotinylated cRNA were prepared from 500 ng of total RNA according to the standard protocol (TotalPrep RNA Amplification Kit, Instruction Manual, Catalog #IL1791, Illumina).
 
Hybridization protocol 1.5 ug of cRNA were hybridized to Illumina Mouse-6 Expression BeadChip v1.1 for 20 hr at 58oC on Ilumina Hybridization Oven.
Scan protocol Illumina Mouse-6 Expression BeadChip v1.1 were washed and stained with streptavidin-Cy3 before scanning using the Illumina Bead Reader.
Description Gene expression data from mouse iPS cells
Data processing The data were analyzed using Bead Array suite software. Rank invariant normalization method was chosen to obtain the signal intensity.
 
Submission date Oct 15, 2008
Last update date Jan 15, 2009
Contact name Vinsensius B Vega
E-mail(s) vegav@gis.a-star.edu.sg
Organization name Genome Institute of Singapore
Street address 60 Biopolis Street
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platform ID GPL6105
Series (2)
GSE13190 Global gene-expression analyses of the Esrrb reprogrammed cells and Esrrb knockdown cells
GSE13211 Global gene-expression analyses of the Esrrb reprogrammed cells

Data table header descriptions
ID_REF Illumina probe ID
VALUE Signal intensity
DETECTION P-VALUE detection p-value

Data table
ID_REF VALUE DETECTION P-VALUE
ILMN_2417611 34.24206 0.9232258
ILMN_1238136 46.08404 0.2825806
ILMN_2721178 450.3509 0
ILMN_2458840 40.59103 0.5741935
ILMN_1230777 1274.745 0
ILMN_2537239 832.1459 0
ILMN_1246069 2446.852 0
ILMN_2524361 46.87052 0.2470968
ILMN_1243193 686.6306 0
ILMN_1232042 43.30736 0.4174193
ILMN_2543689 530.9016 0
ILMN_1233188 76.33546 0
ILMN_1224596 91.64405 0
ILMN_1233643 40.77679 0.5619355
ILMN_1222139 153.123 0
ILMN_1259789 72.56176 0
ILMN_2773981 690.8978 0
ILMN_2771349 1169.703 0
ILMN_1215157 856.7921 0
ILMN_1216623 733.2945 0

Total number of rows: 46632

Table truncated, full table size 1324 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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