|
Status |
Public on Oct 10, 2009 |
Title |
GFP_rep3 |
Sample type |
RNA |
|
|
Source name |
HES2 infected with GFP overexpressing lentivirus, replicate 3
|
Organism |
Homo sapiens |
Characteristics |
Embryonic Stem Cells
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA samples were prepared using Qiagen RNeasy Micro Kit (Qiagen) and then assayed using the Agilent Systems Bioanalyzer 2100 to ensure that high-quality RNA was used for the array experiments.
|
Label |
Cy3
|
Label protocol |
Targets were prepared from 500 ng total RNA using Illumina TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791)
|
|
|
Hybridization protocol |
llumina Human 6 BeadChips were hybridized, washed, stained and scanned according to Illuminas protocol
|
Scan protocol |
Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
|
Description |
1922146134_E
|
Data processing |
Data were first extracted with Illumina BeadStudio software without normalisation. Data was then uploaded into R, where RMA background adjustment and Quantile normalisation was performed using the BeadExplorer package
|
|
|
Submission date |
Oct 17, 2008 |
Last update date |
Oct 17, 2008 |
Contact name |
Sean Grimmond |
E-mail(s) |
s.grimmond@imb.uq.edu.au
|
Organization name |
University of Queensland
|
Department |
Institute for Molecular Bioscience
|
Lab |
Expression Genomics Lab
|
Street address |
Services Rd
|
City |
St Lucia |
State/province |
Q |
ZIP/Postal code |
4072 |
Country |
Australia |
|
|
Platform ID |
GPL6102 |
Series (1) |
GSE13261 |
Direct comparison of HES2 ESC overexpressing CD30 variant with HES2 overexpressing GFP |
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