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Status |
Public on Aug 29, 2018 |
Title |
PBMC + #1 abs |
Sample type |
RNA |
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|
Source name |
PBMC + #1 abs, co-cultured for 48 hr
|
Organism |
Homo sapiens |
Characteristics |
cell type: PBMC disease state: healthy
|
Treatment protocol |
PBMC + Rat IgG, 100 ng/mL PBMC + #1 abs, 100 ng/mL PBMC + OKT-3, 100 ng/mL
|
Growth protocol |
PBMC, 2 X 10^6/well + Rat IgG, 100 ng/mL, co-cultured for 48 hr PBMC, 2 X 10^6/well + #1 abs, 100 ng/mL, co-cultured for 48 hr PBMC, 2 X 10^6/well + OKT-3, 100 ng/mL, co-cultured for 48 hr
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA of (1) PBMC + Rat IgG, (2) PBMC + #1 abs, and (3) PBMC + OKT-3 were extracted by High Pure RNA Isolation Kit (Roche) after the cells were incubated for 48 hr.
|
Label |
biotin
|
Label protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
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|
|
Hybridization protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
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Scan protocol |
We followed manufacturer's protocol, and used GeneChip® Scanner 3000 7G.
|
Description |
PBMC, collected from a healthy donor + #1 abs, novel T-cell suppressive antibody (another name 20-2b2 antibody, host: Rat)
|
Data processing |
Data acquisition and normalization (SST-RMA) were used Affymetrix® Expression Console™ Software.
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|
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Submission date |
Aug 22, 2018 |
Last update date |
Aug 29, 2018 |
Contact name |
Sunao Sugita |
Organization name |
RIKEN Center for Developmental Biology
|
Department |
The Laboratory for Retinal Regeneration
|
Street address |
2-2-3 Minatojima-minamimachi, Chuo-ku
|
City |
Kobe |
ZIP/Postal code |
650-0047 |
Country |
Japan |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE118925 |
Suppression of human T-cell activation by a novel anti-human CD3 antibody |
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