|
| Status |
Public on Aug 29, 2018 |
| Title |
PBMC + OKT-3 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC + OKT-3, co-cultured for 48 hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
disease state: healthy
|
| Treatment protocol |
PBMC + Rat IgG, 100 ng/mL PBMC + #1 abs, 100 ng/mL PBMC + OKT-3, 100 ng/mL
|
| Growth protocol |
PBMC, 2 X 10^6/well + Rat IgG, 100 ng/mL, co-cultured for 48 hr PBMC, 2 X 10^6/well + #1 abs, 100 ng/mL, co-cultured for 48 hr PBMC, 2 X 10^6/well + OKT-3, 100 ng/mL, co-cultured for 48 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA of (1) PBMC + Rat IgG, (2) PBMC + #1 abs, and (3) PBMC + OKT-3 were extracted by High Pure RNA Isolation Kit (Roche) after the cells were incubated for 48 hr.
|
| Label |
biotin
|
| Label protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
|
| |
|
| Hybridization protocol |
We followed protocol of manual that is GeneChip® WT PLUS Reagent Kit User Manual.
|
| Scan protocol |
We followed manufacturer's protocol, and used GeneChip® Scanner 3000 7G.
|
| Description |
PBMC , collected from a healthy donor + OKT-3, common T-cell suppressive antibody (host: mouse)
|
| Data processing |
Data acquisition and normalization (SST-RMA) were used Affymetrix® Expression Console™ Software.
|
| |
|
| Submission date |
Aug 22, 2018 |
| Last update date |
Aug 29, 2018 |
| Contact name |
Sunao Sugita |
| Organization name |
RIKEN Center for Developmental Biology
|
| Department |
The Laboratory for Retinal Regeneration
|
| Street address |
2-2-3 Minatojima-minamimachi, Chuo-ku
|
| City |
Kobe |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE118925 |
Suppression of human T-cell activation by a novel anti-human CD3 antibody |
|
