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Sample GSM3351774 Query DataSets for GSM3351774
Status Public on Apr 15, 2020
Title patient SPN-12 xenograft 1
Sample type RNA
Source name distal lung stem cells
Organism Homo sapiens
Characteristics cell type: xenograft cells
genotype/variation: wild type
patient: SPN-12
Treatment protocol Not applicable.
Growth protocol Cultured stem cell colony on 3T3 feeder layer.
Extracted molecule total RNA
Extraction protocol For the stem cell colony RNA extraction, we have used Arcturus PicoPure RNA isolation kit and for ALI RNA, we have extracted RNA using Trizol and Qiagen RNeasy kit.
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated.
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Affymetrix GeneChip Scanner 3000 7G.
Description SPN-12-Xeno-1
Data processing Data were processed using Partek genomics suite software and RMA background correction were applied to probe intensities, then the corrected intensities were quantile normalized.
Submission date Aug 22, 2018
Last update date Apr 15, 2020
Contact name Frank McKeon
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
Platform ID GPL5175
Series (1)
GSE118950 Pathogenic Stem Cell Variation in Chronic Lung Disease

Data table header descriptions
VALUE Normalized signal

Data table
3881686 6.14914
2672712 6.76818
2842570 5.05378
3526544 4.27717
2902531 5.27556
2402942 6.80333
3382216 5.31536
3771800 6.84888
2427469 5.03828
2392945 6.84911
2453006 8.10943
3552083 5.88221
3416651 5.22234
2562821 7.6419
3721851 6.02201
3442176 6.29904
2477438 4.74818
2926969 5.17958
3026969 6.26211
3796335 5.66657

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.

Supplementary file Size Download File type/resource
GSM3351774_SPN-12-Xeno-1.CEL.gz 21.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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