NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM3351775 Query DataSets for GSM3351775
Status Public on Apr 15, 2020
Title patient SPN-12 xenograft 2
Sample type RNA
 
Source name distal lung stem cells
Organism Homo sapiens
Characteristics cell type: xenograft cells
genotype/variation: wild type
patient: SPN-12
Treatment protocol Not applicable.
Growth protocol Cultured stem cell colony on 3T3 feeder layer.
Extracted molecule total RNA
Extraction protocol For the stem cell colony RNA extraction, we have used Arcturus PicoPure RNA isolation kit and for ALI RNA, we have extracted RNA using Trizol and Qiagen RNeasy kit.
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated.
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Affymetrix GeneChip Scanner 3000 7G.
Description SPN-12-Xeno-2
Data processing Data were processed using Partek genomics suite software and RMA background correction were applied to probe intensities, then the corrected intensities were quantile normalized.
 
Submission date Aug 22, 2018
Last update date Apr 15, 2020
Contact name Frank McKeon
E-mail(s) mckeon.xian@gmail.com
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
 
Platform ID GPL5175
Series (1)
GSE118950 Pathogenic Stem Cell Variation in Chronic Lung Disease

Data table header descriptions
ID_REF
VALUE Normalized signal

Data table
ID_REF VALUE
3881686 6.23852
2672712 6.68596
2842570 5.26124
3526544 4.19108
2902531 5.3755
2402942 6.69916
3382216 5.42927
3771800 6.77605
2427469 5.39971
2392945 7.12563
2453006 7.97562
3552083 5.86366
3416651 4.94858
2562821 7.50207
3721851 6.04073
3442176 6.3664
2477438 4.72034
2926969 4.87639
3026969 6.01564
3796335 5.68384

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.




Supplementary file Size Download File type/resource
GSM3351775_SPN-12-Xeno-2.CEL.gz 21.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap