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Sample GSM3351787 Query DataSets for GSM3351787
Status Public on Apr 15, 2020
Title patient SPN-13 xenograft B14
Sample type RNA
 
Source name distal lung stem cells
Organism Homo sapiens
Characteristics cell type: xenograft cells
genotype/variation: wild type
patient: SPN-13
Treatment protocol Not applicable.
Growth protocol Cultured stem cell colony on 3T3 feeder layer.
Extracted molecule total RNA
Extraction protocol For the stem cell colony RNA extraction, we have used Arcturus PicoPure RNA isolation kit and for ALI RNA, we have extracted RNA using Trizol and Qiagen RNeasy kit.
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated.
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Affymetrix GeneChip Scanner 3000 7G.
Description SPN-13-Xeno-B14
Data processing Data were processed using Partek genomics suite software and RMA background correction were applied to probe intensities, then the corrected intensities were quantile normalized.
 
Submission date Aug 22, 2018
Last update date Apr 15, 2020
Contact name Frank McKeon
E-mail(s) mckeon.xian@gmail.com
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
 
Platform ID GPL5175
Series (1)
GSE118950 Pathogenic Stem Cell Variation in Chronic Lung Disease

Data table header descriptions
ID_REF
VALUE Normalized signal

Data table
ID_REF VALUE
3881686 6.57261
2672712 6.6489
2842570 5.49319
3526544 4.2578
2902531 4.94703
2402942 6.56887
3382216 4.99883
3771800 6.85002
2427469 5.70482
2392945 6.72179
2453006 9.2197
3552083 5.90239
3416651 5.16269
2562821 7.84914
3721851 5.97547
3442176 6.58697
2477438 5.1035
2926969 4.93063
3026969 6.10588
3796335 5.49856

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.




Supplementary file Size Download File type/resource
GSM3351787_SPN-13-Xeno-B14.CEL.gz 21.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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