|
| Status |
Public on Apr 15, 2020 |
| Title |
patient SPN-13 xenograft B5 |
| Sample type |
RNA |
| |
|
| Source name |
distal lung stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: xenograft cells
genotype/variation: wild type
patient: SPN-13
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Cultured stem cell colony on 3T3 feeder layer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For the stem cell colony RNA extraction, we have used Arcturus PicoPure RNA isolation kit and for ALI RNA, we have extracted RNA using Trizol and Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
SPN-13-Xeno-B5
|
| Data processing |
Data were processed using Partek genomics suite software and RMA background correction were applied to probe intensities, then the corrected intensities were quantile normalized.
|
| |
|
| Submission date |
Aug 22, 2018 |
| Last update date |
Apr 15, 2020 |
| Contact name |
Frank McKeon |
| E-mail(s) |
mckeon.xian@gmail.com
|
| Phone |
8608372486
|
| Organization name |
University of Houston
|
| Department |
Biology and Biochemistry
|
| Street address |
3455 Cullen Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77004 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE118950 |
Pathogenic Stem Cell Variation in Chronic Lung Disease |
|
