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Sample GSM3351790 Query DataSets for GSM3351790
Status Public on Apr 15, 2020
Title patient SPN-13 xenograft B6
Sample type RNA
Source name distal lung stem cells
Organism Homo sapiens
Characteristics cell type: xenograft cells
genotype/variation: wild type
patient: SPN-13
Treatment protocol Not applicable.
Growth protocol Cultured stem cell colony on 3T3 feeder layer.
Extracted molecule total RNA
Extraction protocol For the stem cell colony RNA extraction, we have used Arcturus PicoPure RNA isolation kit and for ALI RNA, we have extracted RNA using Trizol and Qiagen RNeasy kit.
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated.
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials.
Scan protocol Affymetrix GeneChip Scanner 3000 7G.
Description SPN-13-Xeno-B6
Data processing Data were processed using Partek genomics suite software and RMA background correction were applied to probe intensities, then the corrected intensities were quantile normalized.
Submission date Aug 22, 2018
Last update date Apr 15, 2020
Contact name Frank McKeon
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
Platform ID GPL5175
Series (1)
GSE118950 Pathogenic Stem Cell Variation in Chronic Lung Disease

Data table header descriptions
VALUE Normalized signal

Data table
3881686 6.5726
2672712 6.91177
2842570 6.07605
3526544 4.21027
2902531 5.1183
2402942 6.90598
3382216 5.15476
3771800 7.36155
2427469 5.00627
2392945 6.71899
2453006 7.00296
3552083 6.17912
3416651 5.27565
2562821 7.99785
3721851 5.93734
3442176 6.52939
2477438 5.67864
2926969 4.82816
3026969 5.30214
3796335 5.67198

Total number of rows: 22011

Table truncated, full table size 341 Kbytes.

Supplementary file Size Download File type/resource
GSM3351790_SPN-13-Xeno-B6.CEL.gz 21.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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