|
| Status |
Public on Oct 22, 2008 |
| Title |
NK cells_Spleen_II |
| Sample type |
RNA |
| |
|
| Source name |
Mouse NK cells sorted from spleen
|
| Organism |
Mus musculus |
| Characteristics |
Age: 12 weeks
Gender: Male
Strain for C57BL/6: RAG-2-KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro Kit
|
| Label |
biotin
|
| Label protocol |
Biotyilated cRNA were generated using Illumina TotalPrep-96 RNA Amplification Kit following manufacturer specifications
|
| |
|
| Hybridization protocol |
Labeled cRNA (1.5µg) were hybridized overnight to Illumina Mouse WG6 V1.1 BeadChip array, washed, blocked, and stained following manufacturer's protocols.
|
| Scan protocol |
BeadChip arrays were scanned on an Illumina BeadStation 500 following manufacturer's specifications. BeadStudio V2 software was used to generate signal intensity values from scans, subtract background, and scale each microarray to the median average intensity for all samples.
|
| Description |
NK cells sorted as CD45+ CD3- Nkp46+ NK11+ from spleen tissue
|
| Data processing |
The data were analyzed using BeadStudio version 1.5.1.3 using the average chip normalization method
|
| |
|
| Submission date |
Oct 20, 2008 |
| Last update date |
Oct 22, 2008 |
| Contact name |
Damien Chaussabel |
| E-mail(s) |
DChaussabel@benaroyaresearch.org
|
| Organization name |
Baylor Institute for Immunology Research
|
| Street address |
3434 Live Oak
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE13151 |
Differential roles of ROR gamma-t in the development of NKp46+ spleen (LTIL) cells and NKp46+ NK cells in gut and skin |
|
