|
| Status |
Public on May 21, 2020 |
| Title |
H838-12V-2 |
| Sample type |
RNA |
| |
|
| Source name |
H838 cancer cell KRAS G12V -2
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: adenocarcinoma
cell type: H838 KRAS G12V cells
|
| Growth protocol |
cell were growth in RPMI1640+10%FBS+L-glutamine+1% PSA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The extraction of total RNA was performed according to the NucleoSpin® RNA kit (Macherey-Nagel) manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
300 ng total RNA were used for cDNA synthesis, labeled by in vitro transcription followed by fragmentation following the manufactory’s suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix).
|
| |
|
| Hybridization protocol |
11 ug labeled samples were hybridized to Clariom D chip at 45℃ for 16.5 hours.
|
| Scan protocol |
The wash and staining were performed by Fluidic Station-450 and the the Clariom D chip were scanned with Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression data from H838 KRAS G12V cells
|
| Data processing |
Generated CEL files were analyzed using Affymetrix Expression Console v. 1.4 and the SST-RMA method. These files in turn are used with the Affymetrix Transcriptome Analysis Console (TAC) Software to produce the differential expression tables and charts.
|
| |
|
| Submission date |
Aug 28, 2018 |
| Last update date |
May 21, 2020 |
| Contact name |
Peishan Hung |
| E-mail(s) |
d99453003@ntu.edu.tw
|
| Phone |
23123456
|
| Organization name |
Graduate Institute of Oncology
|
| Street address |
NO.1 JEN AI ROAD SECTION 1 TAIPEI 100 TAIWAN R.O.C.
|
| City |
Tapei |
| State/province |
State... |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE119146 |
Expression data of H838 KRAS mutation isogenic cell lines |
|
