|
| Status |
Public on Mar 01, 2009 |
| Title |
p53-null Nanog-GFP MEF |
| Sample type |
RNA |
| |
|
| Source name |
p53-null MEF
|
| Organism |
Mus musculus |
| Characteristics |
p53-null mouse embryonic fibroblasts, containing Nanog-GFP reporter
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Trizol.
|
| Label |
Cy3
|
| Label protocol |
Performed as described in One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) Protocol (Agilent, Version 5.7, March 2008).
|
| |
|
| Hybridization protocol |
Performed as described in One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) Protocol (Agilent, Version 5.7, March 2008).
|
| Scan protocol |
Performed as described in One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling) Protocol (Agilent, Version 5.7, March 2008).
|
| Description |
Origin of iPS cells
|
| Data processing |
Data were analyzed with GeneSpring GX 7.3.1. The parameters for normalization are as follows:
Data transformation: Set measurement less than 0.01 to 0.01
Per chip: Normalize to 50th percentile
|
| |
|
| Submission date |
Oct 23, 2008 |
| Last update date |
Oct 27, 2008 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE13312 |
Role of p53 in mouse iPS cell generation |
| GSE13365 |
Effect of p53 on iPS cell generation |
|
