|
| Status |
Public on Aug 31, 2009 |
| Title |
gd T cells from calf 303 stimulated with ultrapure LPS |
| Sample type |
RNA |
| |
|
| Source name |
Bovine gamma delta T cells
|
| Organism |
Bos taurus |
| Characteristics |
gamma delta T cells
from bovine calves
|
| Treatment protocol |
Sorted cells were rested overnight then stimulated for 4 hours with either PBS, Yamoa (32.6ug/ml) or uLPS (10ug/ml).
|
| Growth protocol |
Peripheral blood was collected from 1 to 6 month old calves and gamma delta T cells were sorted to >97% purity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix scanner 7G.
|
| Description |
Gene expression data from stimulated bovine gamma delta T cells
303_uLPS
|
| Data processing |
The data were imported into Genespring using RMA preprocessing for analysis.
|
| |
|
| Submission date |
Oct 23, 2008 |
| Last update date |
Aug 31, 2009 |
| Contact name |
Jodi Fern Hedges |
| E-mail(s) |
jodi.hedges@gmail.com
|
| Phone |
406-994-6384
|
| Organization name |
Montana State University
|
| Department |
Veterinary Molecular Biology
|
| Lab |
Jutila
|
| Street address |
960 Technology Blvd.
|
| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59718 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE13320 |
Changes in gene expression in gamma delta T cells induced by Yamoa |
|
