|
| Status |
Public on Mar 30, 2009 |
| Title |
mouse ES cell-1 vs MEF |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mouse ES cell-1
|
| Organism |
Mus musculus |
| Characteristics |
The mouse ES cells were maintained on irradiated primary murine embryonic feeders (PMEF).
|
| Treatment protocol |
untreated
|
| Growth protocol |
Mouse ES cells were cultured in 85% Knockout DMEM (Invitrogen), 15% defined FBS , 1× MEM nonessential amino acids (Invitrogen), 1× L-glutamine (Invitrogen), 0.1 mM 2-mercaptoethanol , leukemia inhibitory factor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
| Label |
Cy3
|
| Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| |
|
| Channel 2 |
| Source name |
MEF
|
| Organism |
Mus musculus |
| Characteristics |
irradiated primary murine embryonic feeders
|
| Treatment protocol |
untreated
|
| Growth protocol |
MEF was maintained in DMEM (GIBICO) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT), 0.1 mM non-essential amino acids.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
|
| Label |
Cy5
|
| Label protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| |
|
| |
| Hybridization protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| Scan protocol |
Please see manufacturer's web site at http://www.agilent.com/
|
| Description |
This is one of the three repeated sample data.
|
| Data processing |
The microarray was scanned by Agilent G2565BA scanner,raw data were extracted by Feature Extraction Software. Signal intensity were normalized using Lowess method ,GeneSpring 10.0 . VALUE:Cy3/Cy5 signal intensity, ratio:Log2 transformed
|
| |
|
| Submission date |
Oct 27, 2008 |
| Last update date |
Mar 30, 2009 |
| Contact name |
Jing Liao |
| E-mail(s) |
jliao@sibs.ac.cn
|
| Phone |
86-21-54921387
|
| Fax |
86-21-54921388
|
| Organization name |
Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Science
|
| Department |
Institute of Biochemistry and Cell Biology
|
| Lab |
LeiXiao's Lab
|
| Street address |
320 Yue Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE13358 |
Mouse ES cells' gene expression difference compared with that of the MEF cells |
| GSE13462 |
Comparison of human ES cells, mouse ES cells, rat iPS cells and their somatic cells. |
|
