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Status
Public on May 02, 2004
Title
EPC-80hrs-2
Sample type
RNA
Source name
purified erythroid progenitor cells from splenocytes, 80 hours after TAP withdrawel
Organism
Mus musculus
Extracted molecule
total RNA
Description
Developmentally synchronized erythroid progenitor cells (EPCs) were prepared from splenocytes at 80,100 and 120 hours after Thiamphenical withdrawal (as stages I-III), and purified via MACS. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix. This is the 2nd replicate hybridization with stage I EPCs (Kit +++/ EpoR+++ /Ter119(-)neg, late CFU-e). Array: Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A Keywords = erythroid progenitor erythropoiesis profiling
Submission date
Dec 25, 2002
Last update date
May 28, 2005
Contact name
Diya Zhang
E-mail(s)
WOJCHD@mmc.org , diya@northwestern.edu
Phone
(207) 885-8258
Fax
(207) 885-8179
URL
http://www.mmcri.org
Organization name
Maine Medical Center Research Institute
Department
Molecular Medicine
Lab
Dr. Don M. Wojchowski
Street address
81 Research Drive
City
Scarborough
State/province
ME
ZIP/Postal code
04074
Country
USA
Platform ID
GPL81
Series (1)
GSE135
Erythroid Progenitor Cell Gene Expression Profiles
Data table header descriptions
ID_REF
VALUE
Signal output for each probeset from GeneChip 5.0 algorithm
ABS_CALL
Detection call (absent, present, marginal) from GeneChip 5.0 algorithm
P_VALUE
Detection P-value between signals from PerfectMatch probes and MisMatch probes
Data table
ID_REF
VALUE
ABS_CALL
P_VALUE
AFFX-MURINE_B2_at
124099.4
P
0.00003
AFFX-b-ActinMur/M12481_5_at
33702.8
P
0.000044
AFFX-CreX-3_at
67485.2
P
0.000044
AFFX-b-ActinMur/M12481_M_at
54831
P
0.000044
AFFX-CreX-5_at
42515.5
P
0.000044
AFFX-GapdhMur/M32599_3_at
39677.1
P
0.000044
AFFX-b-ActinMur/M12481_3_at
75625.5
P
0.000044
AFFX-GapdhMur/M32599_5_at
26917.1
P
0.000044
AFFX-TransRecMur/X57349_3_at
1531.3
P
0.000095
AFFX-MUR_b2_at
6020
P
0.00004
AFFX-BioC-5_at
5206.1
P
0.000169
AFFX-BioDn-3_at
27124
P
0.000127
AFFX-GapdhMur/M32599_M_at
32189.6
P
0.000052
96255_at
1848.4
P
0.000219
99149_at
1363.2
P
0.000219
94275_at
4900.3
P
0.000219
103340_at
10816.4
P
0.000219
102770_at
12155.3
P
0.000219
100440_f_at
9007.2
P
0.000219
99457_at
7195.4
P
0.000219
Total number of rows: 12488 Table truncated, full table size 324 Kbytes .Supplementary data files not provided