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Sample GSM3373

Query DataSets for GSM3373

Status

Public on May 02, 2004

Title

EPC-100hrs-1

Sample type

RNA

Source name

purified erythroid progenitor cells from splenocytes, 100 hours after TAP withdrawel

Organism

Mus musculus

Extracted molecule

total RNA

Description

Developmentally synchronized erythroid progenitor cells (EPCs) were prepared from splenocytes at 80,100 and 120 hours after Thiamphenical withdrawal (as stages I-III), and purified via MACS. Total RNA preparation, cRNA labeling and hybridization were performed according to recommendations by Affymetrix.
Here is the 1st replicate hybridization of stage II EPCs (Kit+/EpoR+++/Ter119+++/high FALS).
Array: Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A
Keywords = erythroid progenitor erythropoiesis profiling

Submission date

Dec 25, 2002

Last update date

May 28, 2005

Contact name

Diya Zhang

E-mail(s)

WOJCHD@mmc.org , diya@northwestern.edu

Phone

(207) 885-8258

Fax

(207) 885-8179

URL

http://www.mmcri.org

Organization name

Maine Medical Center Research Institute

Department

Molecular Medicine

Lab

Dr. Don M. Wojchowski

Street address

81 Research Drive

City

Scarborough

State/province

ZIP/Postal code

04074

Country

USA

Platform ID

GPL81

Series (1)

GSE135

Erythroid Progenitor Cell Gene Expression Profiles

Data table header descriptions
ID_REF
VALUE	Signal output for each probeset (from GeneChip 5.0 algorithm)
ABS_CALL	Detection call (absent, present, marginal) from GeneChip 5.0 algorithm
P_VALUE	Detection P-value between signals from PerfectMatch probes and MisMatch probes

Data table
ID_REF	VALUE	ABS_CALL	P_VALUE
AFFX-MURINE_B2_at	103681.2	P	0.00003
AFFX-b-ActinMur/M12481_5_at	36786.6	P	0.000044
AFFX-CreX-3_at	64765.5	P	0.000044
AFFX-b-ActinMur/M12481_M_at	45290.8	P	0.000044
AFFX-CreX-5_at	42224.8	P	0.000044
AFFX-GapdhMur/M32599_3_at	5918.2	P	0.000044
AFFX-b-ActinMur/M12481_3_at	47935.6	P	0.000052
AFFX-GapdhMur/M32599_5_at	7315.4	P	0.00007
AFFX-TransRecMur/X57349_3_at	29129.6	P	0.00006
AFFX-MUR_b2_at	5834.2	P	0.000239
AFFX-BioC-5_at	5171.6	P	0.000169
AFFX-BioDn-3_at	21319	P	0.000169
AFFX-GapdhMur/M32599_M_at	8031.8	P	0.000581
96255_at	14185.7	P	0.000219
99149_at	7309.8	P	0.000219
94275_at	26070	P	0.000219
103340_at	53745	P	0.000219
102770_at	56533.1	P	0.000219
100440_f_at	38632.1	P	0.000219
99457_at	36312.5	P	0.000219

Total number of rows: 12488

Table truncated, full table size 325 Kbytes.

Supplementary data files not provided