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Sample GSM3378550 Query DataSets for GSM3378550
Status Public on Jan 01, 2019
Title 107B: nephroureterectomy from patient 118
Sample type RNA
 
Source name upper tract urothelial carcinoma
Organism Homo sapiens
Characteristics tissue preparation: FFPE
tissue: upper tract urothelial carcinoma
grade/ invasive: high/ invasive
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Ambion RecoverAll Total Nucleic Acid Isolation Kit for FFPE following the manufacturer's recommendations
Label SYBR Green
Label protocol cDNA and RT-PCR were performed using the miRCURY LNA miRNA PCR Human Arrays I&II, currently provided by Qiagen formerly from Exiqon, following the manufacturer's recommendations. Catalog number for the array is: 339322
 
Hybridization protocol n/a
Scan protocol n/a
Description sample name: 107B
Data processing Non-normalized data are the Cq values computed by the Bio-Rad CFX themal cycler software, no adjustment to baseline backgrounds were made
Each microRNA of interest was normalized to the global mean within each sample. The global mean was calculated only using microRNA with a Cq<37 detected in all 35 samples. 2^-deltaCq, where deltaCq = (Cq_Target − Cq_global_mean). All Cq values >37 were set to 40.
Fold change (delta delta Ct) values were not computed for this dataset
normalized data matrix reports normalized signal (against each samples global mean).
 
Submission date Sep 06, 2018
Last update date Jan 01, 2019
Contact name Travis Sullivan
E-mail(s) sulliv82@lahey.org
Organization name Lahey Hospital & Medical Center
Street address 41 Mall Rd.
City Burlington
State/province MA
ZIP/Postal code 01805
Country USA
 
Platform ID GPL17107
Series (2)
GSE119601 Micro-RNA expression profiles in upper tract urothelial carcinoma differentiate stage and tumor progression: screening data
GSE119899 Micro-RNA expression profiles in upper tract urothelial carcinoma differentiate stage and tumor progression

Supplementary data files not provided
Processed data are available on Series record

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