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Sample GSM3400840 Query DataSets for GSM3400840
Status Public on Mar 31, 2019
Title Healthy PBMC 2 (P1002_CD4_2)
Sample type SRA
 
Source name Healthy PBMC
Organism Homo sapiens
Characteristics disease state: Healthy
cell type: PBMC
cell subset: CD4 T cells
Extracted molecule total RNA
Extraction protocol Cells were resuspended in TCL buffer, supplemented with 1 mg/mL proteinase K, and processed through the Smart-seq2 protocol (on a microfluidic device)
Libraries were constructed using the Nextera XT (Illumina) library preparation kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 550
 
Description Processed data file: SLE.matrix
Data processing Bcls were demultiplexed using Illumina bcl2fastq (v2.20.0.422)
Fastqs were aligned to hg19 using STAR
Gene expression matrices were generated using Bowtie2
Genome_build: hg19
Supplementary_files_format_and_content: Matrices are provided as samples (columns) vs. genes (rows)
 
Submission date Sep 25, 2018
Last update date May 15, 2019
Contact name Paul Blainey
E-mail(s) pblainey@broadinstitute.org
Organization name Broad Institute
Street address 415 Main St
City Cambridge
State/province Massachusetts
ZIP/Postal code 02142
Country USA
 
Platform ID GPL21697
Series (1)
GSE120442 Multiplexed enrichment and genomic profiling of peripheral blood cells reveal subset-specific immune signatures
Relations
BioSample SAMN10127736
SRA SRX4737452

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data not provided for this record
Raw data are available in SRA

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